Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Purification and characterization of thermostable cellulase from Consortium XM70 in terrestrial hot spring with sugarcane bagasse

Chao Zhao¹, Xiong Lu¹, Yunjin Deng¹, Yifan Huang^1,2 , Bin Liu^1,2 ,

¹College of Food Science, Fujian Agriculture and Forestry University; ²National Engineering Research Center of JUNCAO, Fuzhou 350002, China.

For correspondence:- Yifan Huang Email: zjhyfang@163.com Tel:+8659183530197

Received: 10 November 2014 Accepted: 13 March 2015 Published: 26 April 2015

Citation: Zhao C, Lu X, Deng Y, Huang Y, Liu B, Purification and characterization of thermostable cellulase from Consortium XM70 in terrestrial hot spring with sugarcane bagasse. Trop J Pharm Res 2015; 14(4):591-598 doi: 10.4314/tjpr.v14i4.5

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To better understand the sugarcane bagasse (SCB) degradation process and obtain thermostable cellulase from terrestrial hot spring.

Methods: Molecular community structure of the newly selected thermophilic bacterial consortium XM70 was determined using the method of the full-length 16S rRNA library-based analysis. The thermostable CMCase was purified with ion-exchange and gel filtration chromatography.

Results: Sequence-based identification of species belonging to the genera, Geobacillus, Desulfotomaculum, Bacillus, Exiguobacterium, Paenibacillus and Enterobacter were identified. The maximal activities of carboxymethyl cellulase (CMCase), filter paper cellulase and β-glucosidase of the consortium XM70 were obtained after incubation at 60 °C and pH 6.0 (4 days), 80 °C and pH 7.0 (2 days) and 70 °C and pH 8.0 (4 days), respectively. The yield of reducing sugars in the culture broth achieved 0.11 g.g^-1 dry SCB. An extracellular CMCase from consortium XM70 (XM70-CMCase) was purified 7.9-fold to apparent homogeneity with a recovery of 65.41 % and its molecular mass was about 31.0 kDa. Maximum CMCase activity of the purified XM70-CMCase was 3.77 U/mg at 70 °C and pH 7.0. CMCase activity maintained about its maximal value of 70 % after incubation at 80 °C for 60 min.

Conclusion: Due to its high temperature stability, the purified XM70-CMCase may be useful for industrial application such as biofuel, animal feed industry, paper industry and clarification of fruit juices.

Keywords: Thermostable cellulase, Sugarcane bagasse, Purification, Characterization, Hot spring